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Fig. 8. Disordered 5- to 24-nm-diameter chromatin chains are flexible
and can be packed together at different concentration densities in
interphase nuclei and mitotic chromosomes. (A) Global average of
chromatin diameters in interphase and mitotic EMTdata sets using
continuous erosion analysis. (B) Histogram of subvolume CVC frequencies
in interphase and mitotic cells. (C) In mitotic chromosomes, an individual
chromatin chain is traced, which bends back on itself at short intervals
along its length, forming a compact 3D structure. Left, single TS (1.6 nm
thick); right, rendered chromatin surface; middle, overlay. Scale bar,
40 nm. (D) In interphase cells, an individual chromatin chain is traced, which
has a more extended curvilinear structure than in mitotic chromosomes.
Left, single TS (1.28 nm thick); right, rendered chromatin surface; middle,
overlay. Scale bar, 40 nm. (E) Higher-disorder 3D chromatin packing.
Chromatin is a flexible disordered 5- to 24-nm-diameter granular chain that
is packed together at different 3D volume concentration density distributions
in interphase nuclei and mitotic chromosomes.