Fig. 3. Mouse growth parameters. (A) The proliferation rate determined
from MI in sections is similar (ANOVA, P > 0.05) for all domains except FP.
(B) Fraction of Caspase3+ Sox2+ progenitors. (C) Differentiation rate deter-
mined from transverse sections. (D and E) G1 length relative to the total cell
cycle length (D) estimated from the fraction of S/G2/M-Fucci negative
progenitors (E). Asterisks, one-way ANOVA across domains; P < 0.05. (F)
Neural tube geometry and flat-mount preparation. Colors as in Fig. 1A; labeled
cells, black. (G) Maximum projection though a flat mount, imaged between
somites 6 to 13. Dorsal up, anterior left. Clones marked by EYFP expression.
(H) Coordinates of the EYFP+ cells in (G). Clonal groups are color-coded. (I)
Example clone. Cell coordinates (dots) and typical cell diameter (circles). First
and second eigenvectors of the second moment matrix are shown by red
and blue lines, respectively. Clone orientation angle relative to the anterio-
posterior axis, q. Anterioposterior and dorsoventral clone spread, dashed
lines. (J) The density distribution of pD, pI, and p3 clone orientation angles is
non uniform (c2 test, P < 0.05). (K to M) Mean dorsoventral (K), anterio-
posterior (L) clone spread and ratio of the mean anterioposterior/dorsoventral
spread (M). Asterisks, Student’s t test; P < 0.05. (N) Progenitors in a hemi-
section with anterioposterior length = 1 cell at t = 10 hours and increasing
in three dimensions as calculated from the clonal and section data (see the
supplementary materials). [(A) to (N)] Error bars, mean T SEM. For sample
sizes, see table S2.