Fig. 4. Examples of significant neuron lines and candidate
neurons involved in distinct behaviotypes. (A) Behaviotype
probabilities of negative controls and a few example lines with
sparse expression patterns that were significantly different from
controls. (B to E) Z-projections of confocal stacks of larval nervous
systems illustrating neuronal expression (gold) of selected lines.
Neuropil is stained for reference with antibody to N-cadherin (blue). High-resolution images of candidate neurons from these lines are shown beneath the lower-magnification images of the entire nervous system. These lines biased the probability toward (B) turn-avoid, (C) backup, (D) turn-turn, and (E) escape behaviotype
subfamilies. In some cases, two or more lines that drive in the same neuron biased the probability toward the same behaviotype. R15E06 and R44G12 both drive in
PVL019 (red arrows) and biased the probability toward turn-turn behaviotypes 25, 26, 28, and 29. Ppk, R38A10, and R27H06 drive in the same nociceptor neurons
and biased the probability toward escape behaviotype 13. (F) R82E12 projections (green) overlap tightly with nociceptive axon terminals (red) in a single 0.5-mm
confocal section, likely forming synaptic contacts. These two lines both biased the probability toward the same escape behaviotype 13 and likely belong to the same
anatomical and functional neural circuit.