Microfluidic Electrophoresis Assays
for Rapid Characterization of Protein
in Research and Development
Wednesday, November 14, 2012
12 noon ET, 9 a.m. PT, 4 p.m. UK, 5 p.m. CET
tional modifications or to monitor the purification process of the proteins produces
a large number of samples that can easily exceed the capacity of modern analytical
laboratories. High throughput analytical platforms with high precision, automa-
tion, and ease-of-use are therefore in great demand. Microfluidic-based assays for
screening protein product quality are finding wide use because they address the
limitations of SDS-PAGE as well as other separation assays that depend on conven-
tional capillary electrophoresis. These assays leverage microfluidic technology to
reduce analysis times dramatically compared with conventional techniques, to a
minute or less per sample. In this presentation we will discuss the use of microfluid-
ic-CE platforms for characterization assays such as purity assessment of monoclo-
nal antibodies under reducing and nonreducing conditions, N-glycan profiling, and
determination of protein charge heterogeneity.
Therapeutic proteins are large complex molecules that are often heterogeneous
in molecular structures. The control and characterization of biotherapeutic protein
quality throughout the development process has been a major focus of the biotech-
nology industry. Studies to test the effect of cell culture conditions on posttransla-
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• Learn about the pros and cons of using microfluidics for high throughput
• Obtain expert advice on setting up and validating microfluidics-based assays
• Compare results obtained using microfluidics assays to conventional methods
• Have their questions answered live during the broadcast!
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