Cell Biology is supported by core funding from the Wellcome Trust
(grant 203149). M. T.K. was supported by a Japan Society for the
Promotion of Science KAKENHI grant (16K15095), as well as
research grants from the Mochida Memorial Foundation for
Medical and Pharmaceutical Research, the SGH Foundation, The
Sumitomo Foundation, and The Canon Foundation. Author
contributions: K.S. generated cell lines, established cell
synchronization protocols, performed synchronized time courses,
and performed imaging analyses. J.H.G. and N.N. performed Hi-C
analyses. A.G. and J.N. performed polymer simulations. L.A.M. and
A.G. designed and analyzed coarse-grained models, with A.G.
serving as the lead modeler. A.G. analyzed condensin ChIP data.
L.X. and J.R.P. prepared 1NM-PP1. I.S. performed chromatin-
enriched proteomics analyses. M. T.K. provided constructs for cell
line engineering. A.G., J.H.G., K.S., J.N., W.C.E., L.A.M., and J.D.
designed the project and analyzed the data. All authors contributed
to writing the manuscript. Competing interests: M. T.K. is a
co-inventor on a provisional patent application (PCT/JP2016/059174)
relating to auxin-inducible degron technology filed by the National
Institute of Genetics of Japan. Data and materials availability: All
Hi-C data have been submitted to the Gene Expression Omnibus and
are publicly available (accession number GSE102740).
Materials and Methods
Figs. S1 to S27
Tables S1 to S4
10 August 2017; accepted 9 January 2018
Published online 18 January 2018