NEBNext® Ultra™ II RNA
Library Prep Kits for NGS
Do you need increased sensitivity and specificity
from your RNA-seq experiments? Do you have
ever-decreasing amounts of input RNA? Our
new NEBNext Ultra II RNA kits are optimized
for low ng to µg inputs, have streamlined,
automatable workflows and are available for
directional (strand-specific, using the “dUTP
method”) and non-directional library prep.
Compatible with poly(A) mRNA enrichment or
rRNA depletion, the kits are available with the
option of SPRISelect® beads for size-selection
and clean-up steps.
Visit UltraIIRNA.com to learn more
and request a sample.
Even more from
less for RNA.
One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at firstname.lastname@example.org.
The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
ILLUMINA® and TRUSEQ® are registered trademarks of Illumina, Inc. KAPA™ is a trademark of Kapa Biosystems. AGILEN T® and BIOANALYZER® are registered trademarks of Agilent Technologies, Inc.
© Copyright 2017, New England Biolabs, Inc.; all rights reserved.
NEBNext Ultra II Directional RNA produces the highest yields,
from a range of input amounts
Poly(A)-containing mRNA was isolated from 10 ng, 100 ng and 1 µg of Universal Human
Reference RNA (Agilent® #740000) and libraries were made using the NEBNext Ultra II
Directional RNA Kit (plus the NEBNext poly(A) mRNA Magnetic Isolation Kit), Kapa
Stranded mRNA-Seq Kit, Kapa mRNA HyperPrep Kit and Illumina TruSeq Stranded
mRNA Kit. The input RNA amount and number of PCR cycles are indicated.
Total RNA Input