The Next Generation of
DNA Assembly and Cloning
NEW ENGLAND BIOLABS®, NEB® and NEBUILDER® are registered trademarks of New England Biolabs, Inc.
GIBSON ASSEMBLY® is a registered trademark of Synthetic Genomics, Inc.
While supplies last. Offer valid in the US only. Limit one sample per customer.
The next generation of DNA assembly and
cloning has arrived. With NEBuilder HiFi DNA
Assembly, you’ll enjoy virtually error-free joining
of DNA fragments. More efficient assembly
is now possible, even with larger fragments,
low inputs, or 5´- and 3´-end mismatches.
Additionally, use NEBuilder HiFi to bridge two
dsDNA fragments with a ssDNA oligo. Save time
with less screening or re-sequencing, and benefit
from no licensing fee requirements from NEB
when choosing NEBuilder products.
Request a free sample*
Total size (kb)
NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621)
Gibson Assembly Master Mix (NEB #E2611)
5 x 1 kb frags.
450 bp + 250 bp
NEBuilder HiFi DNA Master Mix offers improved fidelity
over Gibson Assembly Master Mix
Fidelity of assembled products was compared between NEBuilder HiFI DNA Assembly Master Mix
(NEB #E2621) and Gibson Assembly Master Mix (NEB #E2611). Experiments were performed
using the various fragment and vector sizes, following suggested protocols. Experiments B and C vary
because sequences of fragments are different. Experiments D and F were performed with fragments
containing 3´-end mismatches.