NEBNext® Ultra™ II FS DNA Library Prep Kit
Novel Enzymatic Fragmentation System
Do you need a faster, more reliable solution for
DNA fragmentation and library construction?
The NEBNext Ultra II FS DNA Library Prep
Kit meets the dual challenge of generating
high quality next gen sequencing libraries from
ever-decreasing input amounts AND simple
scalability. Enzymatic shearing increases library
yields by reducing DNA damage and sample loss.
Our novel fragmentation reagent is combined
with end repair and dA-tailing reagents, and a
single protocol is used for a wide range of input
amounts (100 pg – 500 ng) and sample types.
You’ll be thrilled to pieces with the result – a reliable,
flexible, high-quality library prep that is fast
thrilled to pieces.
One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc.
For more information, please email us at firstname.lastname@example.org.
The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
ILLUMINA® and NEX TERA® are registered trademarks of Illumina, Inc.
KAPA™ is a trademark of Kapa Biosystems. COVARIS® is a registered trademark of Covaris, Inc.
© Copyright 2017, New England Biolabs, Inc.; all rights reserved.
NEBNext Ultra II FS DNA produces the highest yields,
from a range of input amounts
Libraries were prepared from Human NA19240 genomic DNA using the input amounts and
numbers of PCR cycles shown. For NEBNext Ultra II FS, a 20-minute fragmentation time
was used. For Kapa HyperPlus libraries, input DNA was cleaned up with 3X beads prior to
library construction, as recommended, and a 20-minute fragmentation time was used. Illumina
recommends 50 ng input for Nextera, and not an input range; therefore, only 50 ng was used
in this experiment. “Covaris” libraries were prepared by shearing each input amount in 1X TE
Bufer to an insert size of ~200 bp using a Covaris instrument, followed by library construction
using the NEBNext Ultra II DNA Library Prep Kit (NEB #E7645). Error bars indicate
standard deviation for an average of 3–6 replicates performed by 2 independent users.
to request your sample today.
NEBNext® Ultra™ II FS